Laccase, species

Secondary, quaternary structures

Molecular mass

Optimum pH, substrate

Kinetic parameters^*

Optimum temperature

Tolerance/

Resistance

Reference

EpoA, Streptomyces griseus

2 domains, trimer

114 kDa, (monomer: 38 kDa)

6.5DMPPDA

K_m = 0.42 mM, V_max = 0.85 nmol/min

40˚C

High temperature

[33]

STSL, Streptomyces lavendulae

nd

73 kDa

4.5, catechol

Km ＝ 0.043 mM, k_cat ＝ 10.9 s⁻¹, k_cat/K_m = 253 mM⁻¹∙s⁻¹

50˚C

Alkaline medium, high temperature

[34]

Nd, Streptomyces cyaneus

nd

75 kDa

4.5, ABTS

Km = 0.38 mM

70˚C

High temperature

[19]

SLAC, Streptomyces coelicolor

2 domains, trimer

69 kDa;

(monomer: 32 kDa)

9.4, DMP 4.0, ABTS

Km = 0.4 mM, k_cat = 4 s⁻¹, k_cat/K_m = 10 mM⁻¹∙s⁻¹

60˚C

Alkaline medium, high temperature

[35] [39] [40]

Nd, Streptomyces psammoticus

nd

43 kDa

8.5, ABTS

K_m = 0.39 mM (ABTS), K_m = 3.35 (SGZ)

45˚C

High salt concentration, alkaline medium

[20]

SilA, Streptomyces ipomoea

2 domainsdimer

79 kDa;

(monomer: 44.7 kDa)

5.0, ABTS; 6.5, aromatic amine;

8.0, DMP

K_m = 0.40 mM, V_max = 7.59 mU/µg, k_cat =, 9.99 s^–1, k_cat/K_m = 25 mM^–1∙s^–1 (ABTS); K_m = 4.27 mM, V_max = 3.19 mU/µg, k_cat = 4.20 s^–1, k_cat/K_m = 0.98 mM^–1∙s^–1 (DMP)

60˚C

High salt concentration

[36]

Ssl1, Streptomyces sviceus

2 domains, trimer

98.3 kDa;

(monomer, 32.5 kDa)

4.0, ABTS; 8.0, SGZ; 9.0, DMP or guaiacol;

K_m = 0.36 mM, k_cat = 7.38 s^–1 (ABTS); K_m = 0.89 mM, k_cat=0.32 s^–1 (DMP); K_m = 15.8 mM, k_cat = 5.78 × 10⁻² s^–1 (SGZ)

---

High temperature, alkaline medium, wide pH variation

[21]

SCLAC, Streptomyces sp.

2 domains

38 kDa

8.0, ABTS or guaiacol; 7.0, DMP

K_m = 0.43 mM, V_max = 8.62 mU/µg, k_cat = 8.45 s^–1, k_cat/K_m = 17.6 mM^–1∙s^–1 (ABTS); K_m = 5.58 mM, V_max = 3.45 mU/µg, k_cat = 4.88 s^–1, k_cat/K_m = 0.83 mM^–1∙s^–1 (SGZ); K_m =1.65 mM, V_max = 5.26 mU/µg, k_cat = 2.39 s^–1, k_cat/K_m = 1.45 mM^–1∙s^–1 (guaiacol)

40˚C

Alkaline medium, high temperature

[37]